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Chemical water pollution poses a threat to human beings and ecological systems. (PDF) Aggregation of Rhodamine B in Water - Academia.edu αNF was dissolved in a CH 3 OH/H 2 O mixture (4/1, v/v), and concentrations of stock solutions were calculated using an extinction coefficient (ε 290) of 23,700 M −1 cm −1 (Ueng, Kuwabara, Chun, & Guengerich, 1997).Absorption measurements were recorded with various spectrophotometers, including a Lambda-40 UV-visible spectrometer (Perkin-Elmer, Norwalk, CT), a . This along with a calculated value for the molar extinction coefficient ε of 93879 M −1 cm −1 and pathlength of 1 cm, from single pass measurement of absorbance versus concentration for Rhodamine B, was substituted into eqn (5) to give a CEF of 57.8. Solved The graph below describes the molar extinction | Chegg.com This property makes the dye useful for membrane fusion assays. Other properties listed for this fluorescent dye include the physical form, solubility, melting point, boiling point, pKa, absorption maxima, emission maxima, molar extinction coefficient and quantum yield. Molecular weight: ~ 685. Rhodamine B is associated with eye, skin and respiratory tract irritation. 300 400 500 600 700 800 900 Wavelength (nm) CF532. Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1 ... An amphoteric dye commonly used as a fluorochrome. Use the extinction coefficient ( ɛ) of rhodamine B ( ɛ555 = 106,000) to determine the concentration of the rhodamine B solution. It is used as a biomarker in oral rabies vaccine. Protolytic and associative equilibria in aqueous solutions of rhodamine B were studied.